The present manuscript describes simple, sensitive, rapid, accurate, precise and economical first order derivative spectrophotometry method for the determination of ketoconazole in tablet dosage form. The absorbance value at 256.514 nm of first derivative spectrum was used for the estimation of ketoconazole using methanol as a solvent. This method was found to be linear (r2>0.9989) in the range of 5-35 µg/ml at 256.514 nm. The Limit of detection (LOD) was 0.047 µg/ml. The Limit of quantification (LOQ) was 0.1428 µg/ml. This method was successfully applied for the determination of ketoconazole in tablet dosage form. No interference was observed from excipients present in the tablet. The suitability of this method for the quantitative determination of was ketoconazole proved by validation. The proposed method was found to be simple and sensitive for the routine analysis of ketoconazole in tablet dosage form. The results of analysis have been validated statistically and by recovery studies.
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